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Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators

机译:使用光学微谐振器对自组装脂质双层中Cy5的激发

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摘要

Due to their sensitivity and temporal response, optical microresonators are used extensively in the biosensor arena, particularly in the development of label-free diagnostics and measurement of protein kinetics. In the present letter, we investigate using microcavities to probe molecules within biomimetic membranes. Specifically, a method for self-assembling lipid bilayers on spherical microresonators is developed and the bilayer-nature is verified. Subsequently, the microcavity is used to excite a Cy5-conjugated lipid located within the bilayer while the optical performance of the microcavity is characterized. The emission wavelength of the dye and the optical behavior of the microcavity agree with theoretical predictions.
机译:由于它们的灵敏度和时间响应,光学微谐振器被广泛用于生物传感器领域,特别是在无标记诊断和蛋白质动力学测量方面。在本信中,我们调查使用微腔探测仿生膜内的分子。具体而言,开发了一种在球形微谐振器上自组装脂质双层的方法,并验证了双层性质。随后,使用微腔激发位于双层内的Cy5偶联脂质,同时表征微腔的光学性能。染料的发射波长和微腔的光学行为与理论预测一致。

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